E-viri
PDF
Recenzirano
Odprti dostop
-
Tang, Xu; Zheng, Xuelian; Qi, Yiping; Zhang, Dengwei; Cheng, Yan; Tang, Aiting; Voytas, Daniel F.; Zhang, Yong
Molecular plant, 07/2016, Letnik: 9, Številka: 7Journal Article
The Streptococcus pyogenes CRISPR-Cas9 system effectively mediates RNA-guided DNA double-strand breaks and is used for genome editing in many different organisms, including plants （Puchta, 2016）. CRISPR-Cas9 is a two-component system in which the Cas9 protein is expressed from a Pol II promoter （Lowder et al., 2015）. In contrast, the sgRNAs are typically expressed from Pol III promoters, such as U6 and U3.
