A highly efficient anode is very crucial for an improved microbial fuel cell (MFC) performance. In this study, a binder-free manganese cobalt oxide (MnCo2O4@CF) anode was synthesized using a conventional carbon felt (CF) by a facile hydrothermal method. A large electrochemically active and rough electrode surface area of MnCo2O4@CF anode improved the substrate fluxes and microbial adhesion/growth. Furthermore, the electrochemical tests on the synthesized anode confirmed the superior bioelectrochemical activity, reduced ion transfer resistance, and excellent capacitance. This resulted in an improved power density (945 mW/m2), which was 3.8 times higher than that of CF anode. The variable valence state, high stability and biocompatibility of MnCo2O4@CF resulted in continuous current density performance for five MFC cycles. High-throughput biofilm analysis revealed the enrichment of electricity producing phylum of Proteobacteria and Bacteroidetes (∼90.0%), which signified that the modified MnCo2O4 anode accelerated the enrichment of electro-active microbes. Display omitted •A binderless and stable MnCo2O4@CF anode was fabricated using a facile hydrothermal method.•The synthesized anode generated 3.8 times higher power density than the uncoated electrode.•The improved current density was observed for all five MFC cycles.•Biofilm was enriched with electro-active microorganisms of Proteobacteria and Bacteroidetes.