In this research work, graphene-based highly, conductive, flexible, washable, and breathable textile electrodes have been developed using the pad-dry-cure method. The results show that the electrical conductivity of the graphene-coated textile electrodes was significantly improved from 3.5 MΩ to 400 ± 5.0 kΩ for 12 padding passes. The deposition of poly(3,4-ethylenedioxythiophene) polystyrene sulphonate (PEDOT:PSS) was acquired on reduced graphene oxide (rGO) coated substrate using layer by layer (LbL) technique. The resultant electrodes showed decreased sheet resistance from 150 to 50 kΩ for PEDOT:PSS with a low impedance of 40–150 Hz over the electric potential of 1.0 mV for the detection of high-quality electrocardiograms (ECG) and pulse rate response in different conditions such as rest, walking and running. Consequently, the effect of post-treatment was also analyzed using 5.0 wt% of ethylene glycol (EG) and dimethyl sulfoxide (DMSO), respectively. The sheet resistance was further decreased from 40 kΩ to 130 ±0.5 Ω, which is appropriate for the detection of highly sensitive pulse rate response. The study demonstrates that the pad-dry-cure method can potentially be used for the development of graphene-coated wearable electronic textiles for biomedical and health monitoring devices. Display omitted •Graphene oxide dyeing was accomplished on the woven fabric using pad dry cure method.•The graphene enhanced the electrical conductivity, and tensile strength with the number of dyeing cycles.•The developed nanocomposites improved the electrical conductivity and washing stability against several washes.•The resultant textile electrodes showed better sensor response with higher skin contact and low impedance.