•It is necessary to integrate training on gender-based violence (GV) in the Medicine degree curriculum.•The clinical simulation and problem-based videos are teaching tools that allow the nurses to identify the problem, reflect, and seek solutions.•The debriefing of the cases presented promotes the acquisition of competencies associated to GV.•Learning based on clinical simulation and problem-based videos is effective for improving the competences of medical students in the care of GV victims. Gender-based violence (GV) is a public health problem that affects one out of three women in Spain. The lack of medical training is a barrier to the prevention, protection, and treatment of women who suffer from GV. Training in GV using clinical simulation could be beneficial for medical students. The objective of the present study is to evaluate the knowledge, skills, and attitude concerning GV before and after a training program with clinical simulation and problem-based videos of sixth-year medical students. Quasi-experimental simulation study with measurements before and after GV training with students enrolled in the sixth year of the Medicine degree. An instrument was utilized to measure GV attitude, knowledge, and skills. A final sample of 82 students was obtained. A statistically significant increase (p < .001) was observed in the knowledge, skills, and attitude dimensions. GV training with clinical simulation and problem-based videos provided medical students with knowledge, skills, and attitudes for addressing GV victims. Cite this article: Adánez-Martínez, M. G., et al. (Year, Month). Impact of a training program on gender-based violence of medical students: A quasi-experimental simulation study. Clinical Simulation in Nursing, vol(no.), eXXX-eXXX. doi: 10.1016/j.ecsn.20XX.