Abstract Background and Aims Endoscopic and histological healing are associated with improved clinical outcomes in ulcerative colitis UC. We aimed to investigate the predictive value of faecal immunochemical test FIT for endoscopic and histological healing in UC. Methods We measured quantitative FIT and faecal calprotectin FC in 140 consecutive UC patients who underwent colonoscopy. We assessed the diagnostic accuracy of FIT for predicting endoscopic healing using the Mayo endoscopic subscore MES 0/1 and for histological healing using the Geboes score < 2.0 and Nancy index grade ≤ 1. The predictive abilities of FIT were compared with those of FC. Results FIT had an area under the curve AUC of 0.77 (95% confidence interval CI 0.67–0.86, p < 0.001) for endoscopic healing, an AUC of 0.77 95% CI 0.67–0.86, p < 0.001 using the Geboes score, and 0.77 95% CI 0.66–0.85, p < 0.001 using the Nancy Index for histological healing. The AUC of FIT was comparable to that of FC for endoscopic healing p = 0.773 and histological healing p = 0.767–0.960, and was comparable to colonoscopy for histological healing p = 0.384–0.673. FIT < 50 ng/ml predicted endoscopic healing with a sensitivity, specificity, and positive predictive value PPV of 72%, 68%, and 82%, respectively, and for histological healing with a sensitivity, specificity, and PPV of 73–75%, 67%, and 78–80%, respectively. Combining FIT with FC led to a higher specificity 90% for histological healing. Over 85% of patients with FIT < 50 ng/ml and FC < 50 μg/g achieved histological healing. Conclusions FIT is highly sensitive and accurate to predict endoscopic and histological healing in UC. It represents a promising non-invasive tool for monitoring mucosal healing in UC.