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Generation of two induced pluripotent stem cell (iPSC) lines from p.F508del Cystic Fibrosis patientsFleischer, Aarne; Lorenzo, Iván M.; Palomino, Esther; Aasen, Trond; Gómez, Fernando; Servera, Miguel; Asensio, Víctor J.; Gálvez, Víctor; Izpisúa-Belmonte, Juan Carlos; Bachiller, Daniel
Stem cell research, 20/May , Letnik: 29Journal Article
Cystic Fibrosis (CF) is a monogenic, lethal disease caused by mutations in the cystic fibrosis transmembrane conductance (CFTR) gene. Here we report the production of CF-iPS cell lines from two different p.F508del homozygous female patients (Table 1). Two different primary cell types, skin fibroblasts and keratinocytes, were transfected with retroviral cocktails containing four: c-MYC, KLF4, OCT4 and SOX2 (MKOS) or three: KLF4, OCT4 and SOX2 (KOS) reprogramming factors. Two fibroblast-derived MKOS lines are described in the main text. The lines carry the p.F508del mutation, have a normal karyotype, express pluripotency markers and are able to differentiate into the three germ layers.
