Akademska digitalna zbirka SLovenije - logo
E-viri
Recenzirano Odprti dostop
  • Long Noncoding RNA TARID Di...
    Arab, Khelifa; Park, Yoon Jung; Lindroth, Anders M.; Schäfer, Andrea; Oakes, Christopher; Weichenhan, Dieter; Lukanova, Annekatrin; Lundin, Eva; Risch, Angela; Meister, Michael; Dienemann, Hendrik; Dyckhoff, Gerhard; Herold-Mende, Christel; Grummt, Ingrid; Niehrs, Christof; Plass, Christoph

    Molecular cell, 08/2014, Letnik: 55, Številka: 4
    Journal Article

    DNA methylation is a dynamic and reversible process that governs gene expression during development and disease. Several examples of active DNA demethylation have been documented, involving genome-wide and gene-specific DNA demethylation. How demethylating enzymes are targeted to specific genomic loci remains largely unknown. We show that an antisense lncRNA, termed TARID (for TCF21 antisense RNA inducing demethylation), activates TCF21 expression by inducing promoter demethylation. TARID interacts with both the TCF21 promoter and GADD45A (growth arrest and DNA-damage-inducible, alpha), a regulator of DNA demethylation. GADD45A in turn recruits thymine-DNA glycosylase for base excision repair-mediated demethylation involving oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in the TCF21 promoter by ten-eleven translocation methylcytosine dioxygenase proteins. The results reveal a function of lncRNAs, serving as a genomic address label for GADD45A-mediated demethylation of specific target genes. Display omitted •TARID, a TCF21 antisense lncRNA, is downregulated in cancer cells•TARID binds to the TCF21 promoter and mediates transcriptional activation•TARID recruits GADD45A/TDG, thus promoting base excision-mediated demethylation•A lncRNA can serve as an address label for DNA demethylation The function of most long noncoding RNAs (lncRNAs) is unknown. Arab et al. characterize TARID, a lncRNA that is antisense to the tumor suppressor TCF21, and find that it specifically directs demethylation and activation of TCF21 via GADD45A.