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Tu, Y.-B; Zhou, T; Yuan, X.-F; Qiu, H.-J; Xue, F; Sun, C.-Q; Wang, L; Wu, D.-L; Peng, J.-M; Kong, X.-G; Tong, G.-Z
Archives of virology, 2007, 2007-Jan, 2007-1-00, 20070101, Letnik: 152, Številka: 1Journal Article
The long terminal repeats (LTRs) of equine infectious anemia virus donkey leukocyte-attenuated virus (EIAV-DLA) were substituted with those of the wild-type EIAV-L (wt EIAV-L, the parent virus of EIAV-DLA). The resulting chimeric plasmid was designated pOK-LTR DLA/L. Purified pOK-LTR DLA/L was transfected into monocyte-derived macrophage (MDM) cultures prepared from EIAV-negative, heparinized whole blood from a donkey. Eighth-passage cell cultures developed the typical cytopathogenic effects (CPE) of EIAV infection, and virions with typical EIAV profiles were observed with an electron microscope. Horses were inoculated with the chimeric virus or EIAV-DLA and challenged with the wt EIAV-L strain six months later. All of the horses inoculated with either the chimeric virus or EIAV-DLA were protected from disease, whereas the control horses died with typical EIA symptoms.
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