•n-Butanol does not affect vapour spray penetration even with 60% in volume.•The increase of n-butanol amount decreases drastically the mixture reactivity, so delays the combustion.•Butanol/gasoline blend is able to produce higher efficiency than gasoline fuel under partially premixed combustion mode. As bio-butanol would be one promising alternative fuel to mitigate the energy crisis and reduce the environmental impact of the automotive sector, its potential as blend in gasoline has been widely evaluated especially for conventional Spark Ignition engines. But recent studies prove that Gasoline Compression Ignition operating mode could be a future interesting advanced combustion mode to reach higher efficiency and lower pollutant emissions than conventional SI engines. The objective of this paper is to underline the effect of butanol blend in gasoline in injections conditions needed in GCI by set experimental study in a High Pressure/ High Temperature chamber. Therefore, first data about sprays and combustion characteristics as a function of the butanol content in a gasoline surrogate (PRF80) at injection conditions of GCI, i.e. high pressure (6 MPa) and high-temperature (900 K) conditions but with injection pressure of 40 MPa are given and he results discussed thanks to kinetics simulation of ignition delays.