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Andersen, Jens S; Mann, Matthias
EMBO reports, 09/2006, Letnik: 7, Številka: 9Journal Article
Subcellular organization is yielding to large‐scale analysis. Researchers are now applying robust mass‐spectrometry‐based proteomics methods to obtain an inventory of biochemically isolated organelles that contain hundreds of proteins. High‐resolution methods allow accurate protein identification, and novel algorithms can distinguish genuine from co‐purifying components. Organellar proteomes have been analysed by bioinformatic methods and integrated with other large‐scale data sets. The dynamics of organelles can also be studied by quantitative proteomics, which offers powerful methods that are complementary to fluorescence‐based microscopy. Here, we review the emerging trends in this rapidly expanding area and discuss the role of organellar proteomics in the context of functional genomics and systems biology.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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