•We studied the sol-gel method for the titanium dioxide nanoparticles synthesis from titanyl sulphate.•We studied the synthesis of titanium dioxide and zinc oxide nanoparticles via sol-gel method.•The nanoparticles were synthesized near textile fibers and deposited on them in the same process via ultrasound.•Coated fabrics showed a suppression level of E. coli of more than 99.99% and the antibacterial activity of more than 1.8.•Such textiles can be used to prevent the spread of the nosocomial and other infections. To prevent possible spread of nosocomial infections – HAI (Healthcare Acquired Infections) in healthcare facilities, Antibacterial textiles are developed. This carried out study has been conducted to assess the feasibility of the method of obtaining antibacterial coatings on textile materials. Specifically, the sol-gel method for synthesis of titanium dioxide nanoparticles in combination with zinc oxide nanoparticles from titanyl sulphate and zinc nitrate hexahydrate has been investigated. During the synthesis of titanium dioxide nanoparticles in combination with the zinc oxide nanoparticles, the coated textile material showed stable antibacterial properties with a suppression level ofEscherichia coliof more than 99.99%. The method has been tested on a semi-industrial scale in roll-to-roll experimentby applying homogenous coatings at a speed of 1,5 m per minute.