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Sprotte, Sabrina; Fagbemigun, Olakunle; Brinks, Erik; Cho, Gyu-Sung; Casey, Eoghan; Oguntoyinbo, Folarin A.; Neve, Horst; Mahony, Jennifer; van Sinderen, Douwe; Franz, Charles M.A.P.
Virus research, 01/2022, Letnik: 308Journal Article
•A novel Cequinquevirus PMBT4 was identified from African fermented milk product by electron microscopy and high-throughput sequencing (HTS).•Complete genome sequence of the virus was obtained by HTS.•The Cequinquevirus PMBT4 shares the highest NT identity with Lb. delbrueckii group b phage c5.•No KIS gene element was found in the genome of phage PMBT4 despite the presence of observed collar structures on some phages previously suggested to be encoded by these genes. A novel Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from the Nigerian fermented milk product nono. The phage possesses a long and thin, non-contractile tail and an isometric head, indicating that it belongs to the Siphoviridae family. A neck passage structure (`collar`), previously hypothesized to be encoded by two genes located in the Lactobacillus delbrueckii phage LL-K insertion sequence (KIS) element, as well as in two additional Lb. delbrueckii phages Ld17 and Ld25A, could also be observed on an estimated 1–5% of phage particles by transmission electron microscopy. However, neither mapping of high throughput sequencing data to KIS element genes from Lb. delbrueckii phages LL-K, Ld17 and Ld25A nor PCR amplification of the KIS element genes could corroborate the presence of these genes in the PMBT4 genome. The PMBT4 genome consists of 31,399 bp with a mol% GC content of 41.6 and exhibits high (95–96%) sequence homologies to Lb. delbrueckii phages c5, Ld3, Ld25A and Ld17, which assigned PMBT4 as a new member of this genus, i.e. the Cequinquevirus genus.
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