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Dandey, Venkata P; Budell, William C; Wei, Hui; Bobe, Daija; Maruthi, Kashyap; Kopylov, Mykhailo; Eng, Edward T; Kahn, Peter A; Hinshaw, Jenny E; Kundu, Nidhi; Nimigean, Crina M; Fan, Chen; Sukomon, Nattakan; Darst, Seth A; Saecker, Ruth M; Chen, James; Malone, Brandon; Potter, Clinton S; Carragher, Bridget
Nature methods, 09/2020, Letnik: 17, Številka: 9Journal Article
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.
