Based on the land utilization data of the Lanzhou-Xining urban agglomerations in 2000, 2005, 2010 and 2015, using the methods of transfer matrix, the paper makes a quantitative analysis of the spatial and temporal evolution process, characteristics and laws of various types of national land space from 2000 to 2015. The results are as follows. 1) Urban construction space, industrial and mining construction space and rural living space continued to increase, while agricultural production space showed a decreasing trend, and green ecological space and other ecological space showed an overall increasing trend from 2000 to 2015. 2) The characteristics of national land space transfer are as follows: the transition is relatively active from 2000 to 2005 and from 2010 to 2015, mainly from green ecological space and agricultural production space to urban construction space, rural living space and industrial and mining construction space.