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Rodríguez-Lozano, F. J.; Serrano-Belmonte, I.; Pérez Calvo, J. C.; Coronado-Parra, M. T.; Bernabeu-Esclapez, A.; Moraleda, J. M.
Journal of materials science. Materials in medicine, 04/2013, Letnik: 24, Številka: 4Journal Article
To investigate the effects of new two low-shrinkage composites SDR ® and Venus ® Bulk Fill on the cell viability, cellular damage and expression of mesenchymal markers on dental stem cells. Specimens from two low-shrinkage composites were eluted with culture medium for 24 h. After 24 h of incubation, cytotoxicity of elutes were evaluated by MTT assay; apoptosis was determined using the DNA-specific fluorochrome Hoechst 33342 and the mesenchymal stem cells markers expression was analyzed by immunofluorescence staining. After 24 h of cell exposure to each extract media, dental stem cells expressed MSCs markers. The interaction among the material and cell line was not significantly correlated F(1,60) = 2.251, P = 0.39, whereas statistically significant differences among cells lines were observed F(1,60) = 9.157, P = 0.004, being dental pulp stem cells more resistant that periodontal ligament stem cells. Also, we did not find any significant effect between the tested materials F(1,60) = 0.090, P = 0.765. Furthermore, a very low proportion of exposed cells showed condensed or fragmented nuclei, typical of apoptotic cells at 24 h. The results suggest that SDR ® and Venus ® Bulk fill and should be considered when selecting an appropriate resin-based dental restorative material.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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