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Xu, Hua; Hett, Erik C; Gopalsamy, Ariamala; Parikh, Mihir D; Geoghegan, Kieran F; Kyne, Jr, Robert E; Menard, Carol A; Narayanan, Arjun; Robinson, Ralph P; Johnson, Douglas S; Tones, Michael A; Jones, Lyn H
Molecular bioSystems, 01/2015, Letnik: 11, Številka: 10Journal Article
Despite its diverse applications, such as identification of the protein binding partners of small molecules and investigation of intracellular drug-target engagement, photoaffinity labelling (PAL) is intrinsically challenging, primarily due to the difficulty in discovering functionally active photoaffinity probes. Here we describe the creation of a chemoproteomic library to discover a novel photoaffinity probe for DcpS, an mRNA decapping enzyme that is a putative target for Spinal Muscular Atrophy. This library approach expedites the discovery of photoaffinity probes and expands the chemical biology toolbox to include RNA cap-binding proteins.
