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Morani, Marco; Mai, Thanh Duc; Krupova, Zuzana; Defrenaix, Pierre; Multia, Evgen; Riekkola, Marja-Liisa; Taverna, Myriam
Analytica chimica acta, 09/2020, Letnik: 1128Journal Article
This work reports on the development of the first capillary electrophoresis methodology for the elucidation of extracellular vesicles’ (EVs) electrokinetic distributions. The approach is based on capillary electrophoresis coupled with laser-induced fluorescent (LIF) detection for the identification and quantification of EVs after their isolation. Sensitive detection of these nanometric entities was possible thanks to an ‘inorganic-species-free’ background electrolyte. This electrolyte was made up of weakly charged molecules at very high concentrations to stabilize EVs, and an intra-membrane labelling approach was used to prevent EV morphology modification. The limit of detection for EVs achieved using the developed CE-LIF method reached 8 × 109 EV/mL, whereas the calibration curve was acquired from 1.22 × 1010 to 1.20 × 1011 EV/mL. The CE-LIF approach was applied to provide the electrokinetic distributions of various EVs of animal and human origins, and visualize different EV subpopulations from our recently developed high-yield EV isolation method. Display omitted •A new CE-LIF tool for characterization and quantification of EVs was developed.•The new BGE was applied for excellent performance of CE-LIF of EVs.•Intra-membrane labelling was optimized without incurring EV morphology modification.•Separation and detection of EVs from different animal and human origins were implemented.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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