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  • CGN Correlates With the Pro...
    Tian, Zijian; Meng, Lingfeng; Wang, Xin; Diao, Tongxiang; Hu, Maolin; Wang, Miao; Zhang, Yaqun; Liu, Ming; Wang, Jianye

    Frontiers in molecular biosciences, 02/2022, Letnik: 9
    Journal Article

    This study aimed to screen and verify the important prognostic genes related to clear cell renal cell carcinoma (ccRCC) and further analyze their relationship with the immune microenvironment. Gene expression profiles from the TCGA-KIRC, GSE46699, GSE36895, and GSE16449 datasets were utilized to explore differentially co-expressed genes in ccRCC. We screened 124 differentially co-expressed genes using a weighted gene co-expression network and differential gene expression analyses. Univariate and multivariate Cox survival analyses revealed that the expressions of genes , and were independently related to the overall survival of ccRCC patients. Kaplan-Meier survival analysis was performed, and was found to have the strongest correlation with the prognosis of ccRCC patients and was consequently selected for further analyses and experimental verification. The results showed that NK cell activation, resting dendritic cells, resting monocytes, and resting mast cells were positively correlated with expression; CD4 memory activated T cells, regulatory T cells, and M0 macrophages were negatively correlated with expression. Finally, using western blotting and reverse transcription polymerase chain reaction, we verified that the protein level was down-regulated in ccRCC samples, which was consistent with the mRNA levels. was thus identified as diagnosis and prognosis biomarker for ccRCC and is related to the immune microenvironment.