E-viri
Recenzirano
Odprti dostop
-
Deng, Wulan; Lee, Jongjoo; Wang, Hongxin; Miller, Jeff; Reik, Andreas; Gregory, Philip D.; Dean, Ann; Blobel, Gerd A.
Cell, 06/2012, Letnik: 149, Številka: 6Journal Article
Chromatin loops juxtapose distal enhancers with active promoters, but their molecular architecture and relationship with transcription remain unclear. In erythroid cells, the locus control region (LCR) and β-globin promoter form a chromatin loop that requires transcription factor GATA1 and the associated molecule Ldb1. We employed artificial zinc fingers (ZF) to tether Ldb1 to the β-globin promoter in GATA1 null erythroblasts, in which the β-globin locus is relaxed and inactive. Remarkably, targeting Ldb1 or only its self-association domain to the β-globin promoter substantially activated β-globin transcription in the absence of GATA1. Promoter-tethered Ldb1 interacted with endogenous Ldb1 complexes at the LCR to form a chromatin loop, causing recruitment and phosphorylation of RNA polymerase II. ZF-Ldb1 proteins were inactive at alleles lacking the LCR, demonstrating that their activities depend on long-range interactions. Our findings establish Ldb1 as a critical effector of GATA1-mediated loop formation and indicate that chromatin looping causally underlies gene regulation. Display omitted Display omitted ► Chromatin loops can be generated by targeted tethering of select looping factors ► Forced juxtaposition of a distant enhancer and promoter can trigger transcription ► Ldb1 functions as a looping factor ► Chromatin looping could be used to regulate genes for therapeutic intervention Zinc-finger-mediated tethering of the nuclear factor Ldb1 to the native β-globin promoter is sufficient to juxtapose the promoter with the enhancer and activate transcription. Forced chromatin looping by engineered proteins offers a potential therapeutic strategy to modulate gene expression from a given promoter.
Avtor
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.