E-viri
PDF
Recenzirano
Odprti dostop
-
Janssens, Yorick; Verbeke, Frederick; Debunne, Nathan; Wynendaele, Evelien; Peremans, Kathelijne; De Spiegeleer, Bart
Journal of Pharmaceutical Analysis/Journal of pharmaceutical analysis, 02/2018, Letnik: 8, Številka: 1Journal Article
Five different quorum sensing peptides(QSP)were iodinated using different iodination techniques.These iodinated peptides were analyzed using a C18reversed phase HPLC system,applying a linear gradient of water and acetonitrile containing0.1%(m/v)formic acid as mobile phase.Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides,while semi-quantification was performed using total ion current(TIC)spectra.Non-iodinated peptides and mono-and diiodinated peptides(NIP,MIP and DIP respectively)were well separated and eluted in that order.Depending on the used iodination method,iodination yields varied from low(2%)to high(57%).
