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Metzenmacher, Martin; Váraljai, Renáta; Hegedüs, Balazs; Cima, Igor; Forster, Jan; Schramm, Alexander; Scheffler, Björn; Horn, Peter A; Klein, Christoph A; Szarvas, Tibor; Reis, Hennig; Bielefeld, Nicola; Roesch, Alexander; Aigner, Clemens; Kunzmann, Volker; Wiesweg, Marcel; Siveke, Jens T; Schuler, Martin; Lueong, Smiths S
Cancers, 02/2020, Letnik: 12, Številka: 2Journal Article
Early detection of cancer holds high promise for reducing cancer-related mortality. Detection of circulating tumor-specific nucleic acids holds promise, but sensitivity and specificity issues remain with current technology. We studied cell-free RNA (cfRNA) in patients with non-small cell lung cancer (NSCLC; n = 56 stage IV, n = 39 stages I-III), pancreatic cancer (PDAC, n = 20 stage III), malignant melanoma (MM, n = 12 stage III-IV), urothelial bladder cancer (UBC, n = 22 stage II and IV), and 65 healthy controls by means of next generation sequencing (NGS) and real-time droplet digital PCR (RT-ddPCR). We identified 192 overlapping upregulated transcripts in NSCLC and PDAC by NGS, more than 90% of which were noncoding. Previously reported transcripts (e.g., HOTAIRM1) were identified. Plasma cfRNA transcript levels of POU6F2-AS2 discriminated NSCLC from healthy donors (AUC = 0.82 and 0.76 for stages IV and I-III, respectively) and significantly associated ( = 0.017) with the established tumor marker Cyfra 21-1. cfRNA yield and POU6F2-AS transcript abundance discriminated PDAC patients from healthy donors (AUC = 1.0). POU6F2-AS2 transcript was significantly higher in MM ( = 0.044). In summary, our findings support further validation of cfRNA detection by RT-ddPCR as a biomarker for early detection of solid cancers.
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in: SICRIS
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