Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Jiang, Yuan-Yuan; Chai, Yi-Ping; Lu, Min-Hui; Han, Xiu-Li; Lin, Qiupeng; Zhang, Yu; Zhang, Qiang; Zhou, Yun; Wang, Xue-Chen; Gao, Caixia; Chen, Qi-Jun

Genome Biology, 10/2020, Letnik: 21, Številka: 1

Journal Article

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

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Leto	Faktor vpliva		Izdaja		Kategorija		Razvrstitev
Leto	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

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