A similar phalloidin-stained, TNT-like structure was found in TM tissue Figure 1A3. ...TNTs can provide a direct and specific mode of cellular communication over long distances in TM tissue, effectively overcoming difficulties posed by diffusion-based signaling in a fluid environment. ...the effects of the Rho kinase inhibitor, Y27632, which promotes disassembly of actin stress fibers, were also tested. The thicker TNTs contained tubulin in addition to actin, whereas the thinner TNTs did not. Since the molecular and phenotypic characteristics appear to influence the identity of cargoes transported, further studies are needed to measure TNT diameters in GTM cells. ...a closer examination of the molecular components of TNTs in GTM cells and tissue is warranted given that Myo10 protein had a more punctate distribution in glaucomatous TM tissue compared to age-matched control tissue Figure 1B5 and Figure 1B6.