Human testis development in prenatal life involves complex changes in germline and somatic cell identity. To better understand, we profiled and analyzed ∼32,500 single-cell transcriptomes of testicular cells from embryonic, fetal, and infant stages. Our data show that at 6–7 weeks postfertilization, as the testicular cords are established, the Sertoli and interstitial cells originate from a common heterogeneous progenitor pool, which then resolves into fetal Sertoli cells (expressing tube-forming genes) or interstitial cells (including Leydig-lineage cells expressing steroidogenesis genes). Almost 10 weeks later, beginning at 14–16 weeks postfertilization, the male primordial germ cells exit mitosis, downregulate pluripotent transcription factors, and transition into cells that strongly resemble the state 0 spermatogonia originally defined in the infant and adult testes. Therefore, we called these fetal spermatogonia “state f0.” Overall, we reveal multiple insights into the coordinated and temporal development of the embryonic, fetal, and postnatal male germline together with the somatic niche. Display omitted •A transcriptional single-cell atlas of the fetal and postnatal human testes•Somatic niche cell types derive from a common progenitor ∼7 weeks after fertilization•PGCs transition directly into fetal state 0-like cells (state f0) starting at week 14•Fetal somatic niche cell specification precedes the PGC-to-state f0 transition Guo et al. provide a transcriptional cell atlas of the fetal and postnatal human testes. Remarkably, starting from ∼14 weeks postfertilization, fetal primordial germ cells transition to a cell state highly similar to postnatal spermatogonial stem cells. Furthermore, somatic niche specification precedes this transition, which is consistent with guiding fetal germline development.