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Goldberg, Susanne M. D.; Johnson, Justin; Busam, Dana; Feldblyum, Tamara; Ferriera, Steve; Friedman, Robert; Halpern, Aaron; Khouri, Hoda; Kravitz, Saul A.; Lauro, Federico M.; Li, Kelvin; Rogers, Yu-Hui; Strausberg, Robert; Sutton, Granger; Tallon, Luke; Thomas, Torsten; Venter, Eli; Frazier, Marvin; Venter, J. Craig
Proceedings of the National Academy of Sciences - PNAS, 07/2006, Letnik: 103, Številka: 30Journal Article
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730x/ Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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