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Cao, Wenzhi; Wang, Yao; Shi, Min; Hao, Xiaolong; Zhao, Weiwei; Wang, Yu; Ren, Jie; Kai, Guoyin
Frontiers in plant science, 04/2018, Letnik: 9Journal Article
Tanshinones, one group of bioactive diterpenes, were widely used in the treatment of cardiovascular diseases. WRKYs play important roles in plant metabolism, but their regulation mechanism in remains elusive. In this study, one transcription factor was isolated and functionally characterized from . Multiple sequence alignment and phylogenetic tree analysis showed SmWRKY1 shared high homology with other plant WRKYs such as CrWRKY1. was found predominantly expressed in leaves and stems, and was responsive to salicylic acid (SA), methyl jasmonate (MeJA), and nitric oxide (NO) treatment. Subcellular localization analysis found that SmWRKY1 was localized in the nucleus. Over-expression of significantly elevated the transcripts of genes coding for enzymes in the MEP pathway especially 1-deoxy-D-xylulose-5-phosphate synthase ( ) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase ( ), resulted in over fivefold increase in tanshinones production in transgenic lines (up to 13.7 mg/g DW) compared with the control lines. A dual-luciferase (Dual-LUC) assay showed that SmWRKY1 can positively regulate expression by binding to its promoter. Our work revealed that SmWRKY1 participated in the regulation of tanshinones biosynthesis and acted as a positive regulator through activating in the MEP pathway, thus provided a new insight to further explore the regulation mechanism of tanshinones biosynthesis.
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