Background: Positive psychological well-being (PPWB) is associated with improved health outcomes and positive health behaviors in general populations. However, it is not clear if this is so in women with obesity (BMI>30 kg/m2) who can experience poorer health and weight stigma. Thus, it is important to understand the relationships between BMI and multiple aspects of PPWB, health, and internalized weight bias (IWB). Methods: 1001 women > 18 years of age were surveyed using a web-based design. Five constructs of PPWB were measured including, positive emotion, engagement, relationships, meaning, and accomplishment (PERMA). Participants completed the PERMA-profiler (measures: PERMA, an overall well-being score, happiness, plus separate measures of health, loneliness, and negative emotion); and the modified Weight Bias Internalization Scale (WBIS-M). ANOVA was used to compare the five aspects of PERMA and happiness measures between BMI groups. MANCOVA was used to evaluate sources of variation on the significant individual PERMA measures with BMI groups, adjusting for health, IWB and demographic variables that were significantly different between BMI groups. Results: Women with obesity had significantly lower ratings of PERMA, happiness, general health, and higher IWB when compared to women in normal (BMI=18.5-24.99 kg/m2) and overweight (BMI=25-29.9 kg/m2) categories (p<.001). Health emerged as the greatest predictor of all aspects of PERMA accounting for up to 22% of the variance. IWB and BMI accounted for 1-6% of the variance in PERMA scores. Although women with class 3 obesity (BMI>40.0 kg/m2) had significantly lower PERMA scores than those with class 1 (BMI =30.0-34.9 kg/m2), obesity class was determined not to be a significant factor. Instead, health and IWB explained most of the differences. Conclusions: There is a complex relationship between health, IWB and PPWB in women. Further research is needed to understand the possible multidirectional relationship and to guide future interventions related to PPWB for women with obesity.