In the last decades, some metabolic diseases became a serious problem worldwide whose prevalence has been growth at an alarming rate. The exponential increase of this type of disease is highly related with globalization, sedentarism and high intake of processed food rich in saturated fat and cholesterol. Obesity, diabetes mellitus (DM) and non-alcoholic fatty liver disease (NAFLD) are examples of more common metabolic diseases in actual society and appear to be associated with severe metabolic complications such as hyperglycemia, insulin resistance (IR) and dyslipidemia. Inflammatory status and oxidation characteristic of metabolic diseases highlights the importance of antioxidant properties of some endogenous enzymes in the progression of this type of diseases such as paraoxonase-1 (PON1) and apolipoprotein J (ApoJ).PON1 is a 43 kDa glycoprotein express in the liver and can be modulated by various external factors including diet increasing or attenuating its activity and expression. ApoJ is a sulfated heterodimeric protein with a molecular weight of 75-80 kDa. ApoJ is ubiquitously expressed in various tissues including the liver and acts like a cytoprotective chaperone of some antioxidants enzyme named PON1.The objective of this experimental work is to elucidate the antioxidant and anti-inflammatory role of PON1 and ApoJ in metabolic diseases, that can act as a form of diagnostic and/or treatment of inflammatory diseases. For this, in vitro models of insulin resistance will be used.The obtained results suggest that ApoJ protein levels seems to be increased in response to oleic and linoleic acid, while PON1 levels were unchanged. Furthermore, it was observed that ApoJ protein levels tend to decrease in hyperglycemia. Further works are needed to elucidate ApoJ and PON1 roles in metabolic diseases.