In this paper, a novel photoelectrochemical sensor based on doped quantum dots (QDs) without enzymes was designed for direct detection of H 2 O 2 and indirect detection of glucose. First, the fabrication processes of doped QDs based sensors were described. Second, doped QDs based sensors were tested under different applied potentials and light sources to optimize the measurement conditions. Third, the sensing properties of doped QDs on H 2 O 2 were given, which have been compared with the results from other QDs. Cu doped QDs showed better sensing properties. Finally, detection of glucose was achieved by doped QDs based photoelectrochemical sensors. From the experimental results, it can be concluded that the doped QDs based sensors designed by us can be used to detect H 2 O 2 and glucose.