Akademska digitalna zbirka SLovenije - logo
E-viri
Celotno besedilo
Recenzirano
  • Brucellosis in Turopolje pi...
    Spicic, Silvio; Benic, Miroslav; Zdelar-Tuk, Maja; Duvnjak, Sanja; Karakas, Aneta; Kis, Tomislav; Sucec, Ivica; Cvetnic, Zeljko

    Veterinarski arhiv, 03/2016
    Journal Article

    In the period 2008-2011, a program of diagnostics and control of swine brucellosis was conducted in the remaining two farms of the Turopolje pig breed, including a total of 729 samples of porcine blood. Serological diagnostics were carried out by means of the Rose Bengal test (RBT), complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA). Agreement between serological methods was determined by the Kappa test. Almost perfect agreement was found between the results obtained by the CFT and RBT methods (0.764-0.936), while the agreement between the results obtained by RBT and CFT in comparison with the ELISA test results was substantial (0.547-0.767; 0.647-0.843). In addition, in 2009 and 2011, samples from 50 pigs were subjected to bacteriological tests, and a total of 15 isolates were obtained. Bacteriology was conducted with various time delays from the determination of positive serological reactions, and the method efficacy was determined considering the duration of the infection in pigs. The average efficacy of the isolation of Brucella (B.) suis ranged between 10-43.3 % (P = 0.01) and was significantly higher in acute brucellosis. Isolation was successful for all age categories, regardless of their sexual maturity. Molecular identification confirmed B. suis bv. 2 for the first time in this breed. The extensive breeding of Turopolje pigs and daily contact with live reservoirs of the disease agents are the reasons why the disease cannot be completely eradicated according to the test-and-remove principle. A condition for the successful control and eradication of brucellosis in a Turopolje pig herd is to ensure the conditions for individual and permanent supervision of each breeding pig. Key words: swine brucellosis, Brucella suis, Turopolje pig, Croatia U razdoblju od 2008. do 2011. godine proveden je program dijagnostike i kontrole bruceloze u preostala dva uzgoja turopoljskih svinja na ukupno 729 uzoraka krvi. Seroloska dijagnostika provedena je rose bengal testom (RBT), reakcijom vezanja komplementa (RVK) i imunoenzimskim testom (ELISA). Medusobna podudarnost metoda odredena je kappa testom. Izmedu rezultata dobivenih primjenom RVK i RBT utvrdena je vrlo visoka podudarnost (0,764-0,936). Nesto niza, ali jos uvijek visoka podudarnost utvrdena je izmedu rezultata dobivenih RBT i RVK u usporedbi s rezultatima dobivenim ELISA testom (0,547-0,767; 0,647-0,843). Takoder, tijekom 2009. i 2011. godine bakterioloski je pretrazen materijal podrijetlom od 50 svinja te je ukupno izdvojeno 15 izolata. S razlicitim vremenskim odmakom od utvrdenih pozitivnih seroloskih reakcija ucinjena je i bakterioloska pretraga te odredena uspjesnost metode s obzirom na starost infekcije u uzgoju. Prosjecna uspjesnost izdvajanja vrste Brucella (B.) suis varirala je od 10-43,3 % (P = 0,01), te je bila znacajno veca pri akutnoj brucelozi. Izdvajanje uzrocnika bilo je uspjesno u svih dobnih kategorija, bez obzira na spolnu zrelost svinja. Molekularnom identifikacijom prvi je put u ove pasmine potvrdena infekcija vrstom B. suis bv. 2. Ekstenzivni nacin uzgoja turopoljske svinje i svakodnevni kontakt sa zivim rezervoarima uzrocnika odreduju da se bolest ne moze u potpunosti iskorijeniti prema principu pretrazi i ukloni. Preduvjet uspjesne kontrole i eradikacije bruceloze u stadu turopoljskih svinja jest osiguravanje uvjeta individualnog i trajnog nadzora nad svakom rasplodnom svinjom. Kljucne rijeci: bruceloza svinja, Brucella suis, turopoljska svinja, Hrvatska