E-viri
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Muturi, S M; Karanja, L S; Wachira, F N; Wambulwa, M C
African journal of biotechnology, 09/2013, Letnik: 12, Številka: 39Journal Article
Most of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD sub(260)/OD sub(280) ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors.
