Objectives: Reversible addition-fragmentation chain transfer (RAFT) polymerization belongs to the controlled/living radical polymerization methods and is highly efficient in the preparation of well-defined drug and gene delivery vesicles. Methods: Via RAFT Polymerization different amphlphilic block copolymers consisting of poly(N- acryloyl morpholine) or poly(N-isopropytacrylamide) and poly2-hydroxyethyl methacrylate-block-poly( epsilon -caprolactone) have been prepared. Two different trithiocarbonate RAFT agents have been used and AIBN acted as initiator. As fluorescence marker in cell imaging Fluorescein methacrylate was copolymerized in the hydrophobic block. Micelles were formed by dialysis and their hydrodynamic diameter was characterized by dynamic light scattering (DLS). Results We successfully prepared well-defined amphiphilic block copolymers with low polydispersity indices by RAFT polymerization. They are composed of poly(N-acryloyl morpholine) and poly(N-isopropylacrylamide) as hydrophilic part and biodegradable poly2-hydroxyethyl methacrylate-poly( epsilon -caprolactone) in the hydrophobic part The ability of these block copolymers to self assemble into micelles in an aqueous surrounding was determined by DLS. The uptake of the micelles into living cells was shown by fluorescence imaging. Further modification of the block copolymers is possible due to succinimide units in the polymer backbone. Conclusions Based on biodegradable amphiphilic block copolymers we designed new smart drug carriers, which can assemble into micelles. Because of succinimide groups in the hydrophobic part these block copolymers are able to bind drugs with an amino functionality. The fluorescein moiety included in the polymer backbone allows to monitor the cellular drug uptake by fluorescence imaging. Enzymatic degradation studies of the micelles are currently ongoing.