The «gold standard» among biological and synthetic scaffolds for cultivation is the amniotic membrane. Its preparation for the needs of tissue engineering is associated with the difficulties of transporting and preserving the native amniotic membrane. The amniotic membrane was taken after elective caesarean section. The separated amniotic membrane was fixed according to our method 5. Scaffolds were divided into 3 groups of 5 membranes each: storage under hypothermia, cryopreservation at –20 °C and –80 °C. Stem cells of the corneal epithelium of rabbits were used as a test system, and cells cultured under standard conditions were used as a control. Viability was determined using phase contrast microscopy and microtiter test. It has been suggested that the inhibition of the state of cells cultured on the amniotic membrane by the 14th day is associated with the viability of the own cells of the amniotic membrane. To verify this assumption, a microtiter test was carried out for all scaffold groups. The described method of immobilization of the amniotic membrane provides transportation, preservation and the possibility of culturing stem cells on the amniotic membrane. For the cultivation of stem cells during the first day, all three types of preservation of the amniotic membrane are suitable. In order to create bioengineered structures for restoration of the corneal epithelium, further research is needed to find the optimal way to de-epithelialize the amniotic membrane.