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  • Increased ammonium in cultu...
    Virant-Klun, Irma; Tomaževič, Tomaž; Vrtačnik-Bokal, Eda; Vogler, Andrej; Krsnik, Mladen; Meden-Vrtovec, Helena

    Fertility and sterility, 02/2006, Letnik: 85, Številka: 2
    Journal Article

    The objective of this prospective study was to evaluate the effect of ammonium accumulated in sequential media and determined by enzymatic spectrophotometric method on the blastocyst development in 281 human embryos from 100 stimulated and natural in vitro fertilization (IVF) cycles. Ammonium concentration was increased in 62% of cycles and was correlated negatively with the blastocyst development after classical IVF, but not after intracytoplasmic sperm injection (ICSI).