ABSTRACT Low temperature is an environmental stress that affects crop production and quality and regulates the expression of many genes, and the level of a number of proteins and metabolites. Using leaves from pepper (Capsicum annum L.) plants exposed to low temperature (8 °C) for different time periods (1 to 3 d), several key components of the metabolism of reactive nitrogen and oxygen species (RNS and ROS, respectively) were analysed. After 24 h of exposure at 8 °C, pepper plants exhibited visible symptoms characterized by flaccidity of stems and leaves. This was accompanied by significant changes in the metabolism of RNS and ROS with an increase of both protein tyrosine nitration (NO2‐Tyr) and lipid peroxidation, indicating that low temperature induces nitrosative and oxidative stress. During the second and third days at low temperature, pepper plants underwent cold acclimation by adjusting their antioxidant metabolism and reverting the observed nitrosative and oxidative stress. In this process, the levels of the soluble non‐enzymatic antioxidants ascorbate and glutathione, and the activity of the main NADPH‐generating dehydrogenases were significantly induced. This suggests that ascorbate, glutathione and the NADPH‐generating dehydrogenases have a role in the process of cold acclimation through their effect on the redox state of the cell. Low temperature is an environmental stress that affects plants growth and consequently crop production and quality. Pepper plants are a worldwide consumable vegetables affected for this stress. In this work it is studied how low temperature influences the metabolism of reactive oxygen and nitrogen species as well as the redox state being the soluble antioxidants (ascorbate and glutathione) and the NADPH‐generating dehydrogenases key components in the process of cold acclimation.