博士 國立臺灣大學 農業化學研究所 92 In this study, a fungal strain with high quantity of yellow pigment was isolated from an orchard soil from Pingtung in our laboratory. This strain was identified as Penicillium sclerotiorum and was designated as Penicillium sclerotiorum NTU-FC-13. A yellow needle crystal was obtained by silica-gel column chromatography from the crude pigments, and was identified as sclerotiorin through MS, IR, and NMR spectra. The results showed that the potato agar medium was the best for producing pigments among natural mediums. Maltose could significantly increase the pigment production. Neither organic nor inorganic nitrogen source would influence the pigments production but could increase the volume of biomass. Adding 0.1% sodium chloride could promote the productivity. P. sclerotiorum NTU-FC-13 produces sclerotiorin yellow pigment under submerged fermentation in potato maltose medium (15% potato, 2% maltose, 0.1% NaCl ), the sclerotiorin yield is 633 mg L-1 culture fluid and 39 mg g-1dry cell weight