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Alcohol dehydrogenase in non-aqueous media using high-pressure technologies : reaction set-up and deactivation determinationThorey, Paul ; Knez, Željko ; Leitgeb, MajaBACKGROUND: The demand for enantiomerically pure molecules is growing continuously and biocatalysis is a powerful technique for their production. Inthis work, the catalyst was an enzyme combined with ... its coenzyme, NADP. Highpressure technology, a second clean technology, was applied as well. Densegases are promising solvents for biocatalysis. They have been investigated extensively as reaction media for lipase-catalysed reactions, butseldom for reactions, catalysed with alcohol dehydrogenases, as in this work. RESULT: The production of optically pure R-1-phenylethanol from acetophenone was investigated. The NADP-dependent alcohol dehydrogenase from Lactobacillus brevis (LBADH) was used as a catalyst. The hydrogenation was performed with isopropanol as a co-substrate in different conditions: dense propane with LBADH and NADP co-immobilized on glass beads and in the biphasic system water/dense propane. The obtained R-1-phenylethanol was enantiopure. The conversions were up to 90%. Deactivation of LBADH was also measured in these media. CONCLUSION: Protocols were successfully developed for the testing of alcohol dehydrogenase activity in dense gases. Enantioselectivity of LBADH is excellent in those media but it deactivated quickly. An LBADH-catalysed reaction was performed in a dense gas for the first time.Source: Journal of chemical technology and biotechnology. - ISSN 0268-2575 (Vol. 85, 2010, str. 1011-1016)Type of material - article, component partPublish date - 2010Language - englishCOBISS.SI-ID - 14291222
Author
Thorey, Paul |
Knez, Željko |
Leitgeb, Maja
Topics
Lactobacillus brevis |
alcohol dehydrogenase |
deactivation |
immobilization |
high pressure technology |
dense gases |
biphasic system
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source: Journal of chemical technology and biotechnology. - ISSN 0268-2575 (Vol. 85, 2010, str. 1011-1016)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Thorey, Paul | ![]() |
Knez, Željko | 02619 |
Leitgeb, Maja | 00534 |
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