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The in vitro caecal fermentation of different starch sources in rabbits
Kermauner, Ajda ; Lavrenčič, Andrej, 1965-
The objective of our study was to extend the knowledge of microbial fermentation of starch in the rabbit caecum using the in vitro gas production technique. Different sources of starch (wheat and ...maize grain, raw and cooked potato) and different starch isolates (wheat, maize and potato starch) were incubated with inoculum, prepared from rabbit caecum content. The gas production parameters such as total potential gas production (parameter "B"), gas production till 10 h of incubation (Gas10), maximum fermentation rate (MFR) and time of maximum fermentation rate (TMFR) were estimated with Gompertz model and were significantly affected by the substrata. Differences between maize grain and maize starch were small: MFR and TMFR were not different, while Gas10 of maize starch was higher than from maize grain (5 and3 ml/kg DM, respectively; P < 0.05). MFR of wheat starch was higher than that of wheat grain (3.1 and 2.4 ml/h, respectively; P < 0.05), but reached maximum rate later (TMFR: 12.4 and 10.9 h, respectively; P < 0.05) and had lower Gas10 (6 and 9 ml/kg DM, respectively; P < 0.05). The fermentation of cooked potato reached the TMFR after 8.5 h of incubation and had the highest MFR (2.9 ml/h) and Gas10 (16 ml/kg DM) of all substrata. The MFRs of raw potato and potato starch were similar (2.1 and 2.3 ml/h, respectively), although TMFR and Gas10 of raw potato were shorter and higher than those of potato starch (TMFR of 10.7 and 17.2 h and Gas10 of 10 and 1 ml/kg DM, respectively; P < 0.05).
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