-
Izolacija in karakterizacija 6-fosfofruktokinaze pri glivi Aspergillus niger : diplomska naloga = Isolation and characterization of 6-phosphofructokinase from Aspergillus niger : graduation thesisSmerkolj, Sava6-fosfofrukto-1-kinaza je eden kljuřnih regulatornih encimov glikolize. Ćelelismo ugotoviti, ali se pri sevu A60 glive Aspergillus niger pojavlja v veř oblikah in te oblike okarakterizirati. Glivo ... smo gojili v dveh razliřnih gojiǴřih in v enem primeru izvrǴili hipoosmotski Ǵok. Z gelsko filtracijo smo lořili dve po velikosti razliřni obliki PFK1 in ugotovili, da se lořita tudi po kinetiki v odvisnosti od ATP. S pomořjo frakcioniranega obarjanja smo prav tako lořili dve obliki in ugotovili, da se razlikujeta po stanju fosforiliranosti in kinetiki. Z afinitetno kromatografijo smo ořistili obe obliki encima in nato z lořitvijo s poliakrilamidno gelsko elektroforezo dolořili njuni molski masi, ki sta okoli 48 in 84 kDa. Zakljuřili smo, da se verjetno veřja oblika encima fosforilira preko cAMP-odvisne proteinske kinaze,na ta nařin postane nestabilna, razpade do manjǴe oblike in tako tudi spremeni svojo kinetiko.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [S. Smerkolj] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000Language - slovenianCOBISS.SI-ID - 2435704
Author
Smerkolj, Sava
Other authors
Herzog-Velikonja, Blagajana |
Legiša, Matic |
Žgur-Bertok, Darja
Topics
Aspergillus niger |
encimi |
izolacija encimov |
karakterizacija encimov |
gojišča |
fosforilacija |
6-fosfofrukto-1-kinaza |
proteinska kinaza |
proteinska kinaza |
Aspergillus niger |
enzyme isolation |
enzyme characterization |
media |
phosphorilation |
6-phosphofructo-1-kinase |
protein kinase |
protein kinase
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Reserve material at the desired pickup location.
Pickup location |
Material status | Reservation |
---|---|---|
BF, Dept. of Food Science and Technology, Lj. |
available - outside loan, loan period: 1 months
|
Call number – location, accession no. ... |
Copy status |
---|---|
Študij mikrobiologije DN 0000000060 IN: 0012359 Študij mikrobiologije DN 60 IN: 0012359 |
available - outside loan, loan period: 1 months
|
Študij mikrobiologije DN 0000000060 a IN: 0012360 Študij mikrobiologije DN 60 a IN: 0012360 |
available - outside loan, loan period: 1 months
|
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Herzog-Velikonja, Blagajana | 09836 |
Legiša, Matic | 09354 |
Žgur-Bertok, Darja | 07042 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.
Izposojeno gradivo lahko vrnete tudi v nabiralnik pred knjižnico.