BF, Dept. of Animal Science, Ljubljana (BFZOO)
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Osamitev in opis vampnih spirohet = Isolation and characterization of ruminal spirochetesTrkov, Marija ; Accetto, Tomaž ; Avguštin, GorazdIz vzorca vampne vsebine smo osamili tri seve vampnih spirohet. Morfologijo osamljenih sevov smo opazovali s fazno kontrastno in epifluorescentno mikroskopijo ter jih opisali s hitrimi biokemijskimi ... preskusi (API 50CH) in molekulsko biološkimi preiskavami. Rezultati preiskav so pokazali, da sta si seva SM1 in SM3 zelo podobna in značilno različna od seva SM2. Vsi osamljeni sevi so bili Gram negativni. Celice sevov SM1 in SM3 so bile manjše od celic seva SM2, pri sevih SM1 in SM3 je bila krajša tudi amplituda in valovna dolžina posameznega zavoja. Od 49 ogljikovih hidratov so vsi osamljeni sevi razkrajali sedem ogljikovih hidratov, sev SM2 pa je razkrajal še dodatnih 14. Primerjava literaturnih podatkov razkroja ogljikovih hidratov tipskih sevov T. bryantii in T. saccharophilum je pokazala, da se seva spirohet SM1 in SM3 razlikujeta od opisanih vrst, sev SM2 pa je podoben tipskemu sevu T. saccharophilum. Veliko raznolikost med preiskovanimi sevi pa smo ugotovili s teoretično in eksperimentalno preiskavo RFLP, pri čemer smo gen za 16S rRNK sevov T. bryantii, T. saccharophilum, SM1, SM3 in SM2 razrezali z različnimi restrikcijskimi encimi.Source: Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo. Zootehnika = Research reports [of the] Biotechnical Faculty University of Ljubljana. Agricultural issue. Zootechnica. - ISSN 1408-3434 (Letn. 76, št. 2, 2000, str. 181-190)Type of material - article, component partPublish date - 2000Language - slovenianCOBISS.SI-ID - 1079688
Author
Trkov, Marija |
Accetto, Tomaž |
Avguštin, Gorazd
Topics
mikrobiologija |
spirohete |
osamitev |
opis |
vamp |
microbiology |
spirochetes |
isolation |
characterization |
rumen
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Database name | Field | Year |
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Trkov, Marija | 14762 |
Accetto, Tomaž | 19485 |
Avguštin, Gorazd | 08320 |
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and: SICRIS
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