Faculty of Pharmacy, Lj. (FFALJ)
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Primerjava metod UV-VIS spektrofotometrije in pretočne injekcijske analize za določanje hitosana v vodnih raztopinah = Comparison of UV-VIS spectrophotometry and flow injection analysis for determination of chitosan in water solutions : diplomska nalogaŠoštar, BarbaraHitosan je polimer, ki se veliko uporablja na razliènih podroèjih. V farmacijiga uporabljamo predvsem kot pomozno snov z bioadhezivnimi lastnostmi.Deluje tudi protimikrobno.Nasa naloga je bila ... razviti metodo za doloèanje hitosana v vodnih raztopinah, ki omogoèa doloèanje vsebnosti in raztapljanja hitosana v farmacevtskih izdelkih. Detekcija hitosana je tezavna,ker ne absorbira v UV in vidnem obmoèju, njegova razgradnja do monomerov in identifikacija le-teh pa dolgotrajna in potratna. Zato smo sklenili izvesti reakcijo z ninhidrinom in detekcijo produkta z UV-VIS spektrofotometrom. Analizirali smo vsebnost hitosana v tamponih. Ker so bili rezultati, dobljeni z analizo vzorcev, pod mejo detekcije UV-VIS spektrofotometra, smo uporabili se pretoèno injekcijsko analizo. Pretoèno injekcijsko analizo smo izvedli z aparaturo za tekoèinsko kromatografijo brez uporabe kolone. Najprej smo z vodnimi raztopinami pentoksifilina preizkusili, èe je izvedba na nasih aparaturah za tekoèinsko kromatografijo sploh mogoèa inèe daje ta metoda boljse rezultate kot UV-VIS spektrofotometrija. Ugotovili smo, da je metoda linearna, ponovljiva in da ima nizjo mejo kvantifikacije in mejo detekcije kot UV-VIS spektrofotometer. Primerjali smo tudi razliène aparature za tekoèinsko kromatografijo in doloèili, katera aparatura daje najboljse rezultate ter na tej razvili metodo za doloèanje hitosana v 0,1 % raztopini ocetne kisline. Doloèili smo mejo detekcije. Najnizja meja detekcijeznasa 0,4 Žgžml za pentoksifilin in 10 mgžml za derivatiziran hitosan.Razviti smo morali metodo za raztapljanje hitosana, nanesenega na tampone. Analizirali smo celotne tampone, zgornji sloj tamponov, poskusili smos koncentriranjem raztopine vzorca in z metodo s standardnim dodatkom. Preizkusili smo vezavo hitosana na viskozna vlakna. Prouèevali smo tudi topnost hitosana v vodnih medijih z razliènima pH-jema. Tablete hitosana so v kislem okolju nabreknile in se poèasi raztapljale, dokler se niso popolnoma raztopileč v nevtralnem pH-ju pa takoj razpadle in se zelo poèasi in malo raztopile do koncentracije, ki je bila pod mejo detekcije pretoène injekcijske analize.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [Šoštar, B.], 2006Language - slovenianCOBISS.SI-ID - 1912177
Author
Šoštar, Barbara
Other authors
Planinšek, Odon
Topics
hitosan |
pentoksifilin |
raztapljanje |
UV-VIS spektrofotometrija |
analizne metode |
HPLC
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Knjižnica dp 0000000543 Šoštar Barbara Primerjava IN: 0008888 Knjižnica dp 543 Šoštar Barbara Primerjava IN: 0008888 |
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Šoštar, Barbara | ![]() |
Planinšek, Odon | 14935 |
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