Isolation and characterization of Clostridium difficile from children up to 24 months of age = Izolacija in karakterizacija bakterije Clostridium difficile pri otrocih starih do 24 mesecev
Turk, Martina, 1970- ; Rupnik, Maja, 1967-
The prevalence and the epidemiology of Clostridium difficile in stools of hospitalized children aged less than two years was studied. Out of 186 stool specimens received from eight different centers ...in Slovenia, 15 (8.1%) were positive for the C. diffcile culture. From the positive samples, 41 strains were isolated. Thirty-six strains (87%) were toxinogenic and five were not. All the strains were typed by serotyping and polyacrylamide gel electrophoresis of whole cell proteins (PAGE). They were distributed among 8 different serogroups and 7 different PAGE profiles. In the toxinogenic strains, the toxin genes were typed with PCR amplification and enzymatic restrictions. The majority of strains exhibited the same toxinotype 0, except five strains (all from the same stool sample) that were toxinotype VIII. Theseresults suggest that the overall prevalence of C. difficile among hospitalized children is low and that no epidemic strain is observed.
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