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  • Three-dimensional observations on thick biological speciments by high voltage electron microscopy
    Nagata, Tetsuji
    Thick biological specimens prepared as whole mount cultured cells or thick sections from embedded tissues were stained with histochemical reactions, suchas thiamine pyrophosphatase, ... glucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactions and radioautography, to observe 3-D ultrastructures of cell organelles producing stereo-pairs by high voltage electron microscopy at accerelating voltages of 400-1000 kV. The organelles demonstrated were Golgi apparatus, endoplasmic reticulurn, mitochondria, lysosomes, peroxisomes. pinocytotic vesicles and incorporations of radioactivecompounds. As the results, those cell organelles were observed 3-dimensionally and the relative relationships between these organelles were demonstrated.
    Source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 19, no. 1, mar. 2000, str. 51-56)
    Type of material - article, component part
    Publish date - 2000
    Language - english
    COBISS.SI-ID - 11478745

    Link(s):

    Digitalna knjižnica Slovenije - dLib.si

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