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Testiranje učinkov kemičnih retrakcijskih sredstev na celični kulturi fibroblastov = Testing the effects of chemical retraction agents on fibroblast cell cultureKopač, Igor ; Batista, UrškaChemical retraction agents used in fixed prosthodontics have more or less pronounced harmful effect on living cells. The present study using the dye exclusion test, colony forming ability test and ... colorimetric assay was undertaken to evaluate cytotoxic effects of four chemical retraction agents oncultured V-79 fibroblasts, and the dependence of cytotoxicity on the agent concentration and time of exposure. The most aggressive agent, 25 % aluminum chloride, took only one minute to damage all cell cultures. The proportion of cells damaged after 10 minutes of exposure to tetrahydrozoline was 60 %, whichwas significantly less compared to the other chemicals tested. With the colony forming ability test using retraction agents diluted to 1:10, the greatest number of colonies emerged in samples treated with tetrahydrozoline (statistical signifi- cance p < 0.01). The colorimetric assay showed equal cytotoxic effects for 25% aluminum sulphate and tetrahydrozoline. The colorimetric test used in the study has proved an ergonomic, accurate and reliable test for cytotoxicity determination.Source: Zobozdravstveni vestnik. - ISSN 0044-4928 (Letn. 55, št. 4/5, 2000, str. 110-117)Type of material - article, component partPublish date - 2000Language - slovenianCOBISS.SI-ID - 12460249
Author
Kopač, Igor |
Batista, Urška
Topics
Fibroblasts |
Drug effects |
Cells, cultured |
Drug effects |
Cell survival |
Colorimetry |
Tetrazolium salts |
Aluminum compounds |
Chlorides |
Sulfates |
Trypan blue |
Denture, partial, fixed |
Gingiva |
Drug effects |
stomatologija |
fiksna protetika |
dlesni |
retrakcijska sredstva |
kirurgija
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Database name | Field | Year |
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Kopač, Igor | 08224 |
Batista, Urška | 08588 |
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