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  • Vpliv dejavnika tumorske nekroze-alfa na izločanje interlevkina-6 iz človeške mišice v kulturi = The influence of tumor necrosis factor-alpha on the secretion of interleukin-6 from the cultured human muscle
    Prelovšek, Oja
    Tumor necrosis factor-a (TNF-alpha) is a pleiotropic cytokine which, as a partof complex cytokine system, controls the synthesis and expression of othercytokines and their receptors through the ... activation of cytokine cascades. The aim of our study was to test the effects of TNF-alpha on the secretion of interleukin-6 (IL-6) from cultured human muscle. We studied theseeffects before and after the fusion of myoblasts into multinucleated myotubes. Cultures, prepared from satellite cells isolated from the muscle pieces routinely discarded at some orthopaedic operations were treated with TNF-alpha at three different concentrations. We found that TNF-alpha increasesthe IL-6 secretion in a concentration dependent manner. Quantitatively, secretion from myotubes exceeded the secretion from myoblasts indicating that secretion is differentiation stage-dependent. The presence of TNF-alpha receptor was confirmed by Western blot. Apoptosis, followed by DNA fragmentation, remained unchanged. Our results support the concept that skeletal muscles actively participate in cytokine signalling in critical clinical conditions.
    Source: Medicinski razgledi : [medicinski pregledni, strokovni in raziskovalni članki]. - ISSN 0025-8121 (Letn. 43, št. 1, mar. 2004, str. 63-76)
    Type of material - article, component part
    Publish date - 2004
    Language - slovenian
    COBISS.SI-ID - 18190297

    Link(s):

    Digitalna knjižnica Slovenije - dLib.si

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