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Analysis of endoplasmic reticulum of tobacco cells using confocal microscopyRadochová, Barbara ...Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are ... presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement ofER were reconstructed and rendered by various visualization techniques.Source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (no. 3, Vol. 24, nov. 2005, str. 181-185)Type of material - article, component partPublish date - 2005Language - englishCOBISS.SI-ID - 20442073
Author
Radochová, Barbara |
Janáček, Jiří |
Schwarzerová, Kateřina |
Demjénová, Erna |
Tomori, Zoltán |
Karen, Petr |
Kubínová, Lucie
Topics
Tobacco |
Ultrastructure |
Endoplasmic Reticulum |
Microscopy, Confocal |
Image Processing, Computer-Assisted |
Endoplazemski retikulum |
Mikroskopija konfokalna |
Slika, obdelava z računalnikom |
Tobak |
citologija |
stereologija |
mikroskopija |
tobak |
endoplazmatski retikulum
source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (no. 3, Vol. 24, nov. 2005, str. 181-185)
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Radochová, Barbara | |
Janáček, Jiří | |
Schwarzerová, Kateřina | |
Demjénová, Erna | |
Tomori, Zoltán | |
Karen, Petr | |
Kubínová, Lucie |
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