An investigation of the molecular genetics of the gene cluster for oxytetracycline biosynthesis from Streptomyces rimosus : dissertation thesis = Študij molekularne genetike skupine genov za biosintezo oksitetraciklina iz Streptomyces rimosus : doktorska disertacija
Petković, Hrvoje
Celotna skupina genov ya biosinteyo oksitetraciklina (OTC) iy bakterije S. rimosus je Íe klonirana. Eden od nařinov, da se doloři vloga dolořenega gena vskupini genov ya biosinteyo OTC je, da se ...identificira metabolit ali metaboliti, katere producira rekombinantni sev, ki ima ta gen prekinjen (neaktiven). S tem namenom, so najprej rayviti, oyiroma testirani rayliřni postopki ya prekinitev gena. OpaÍena je bila visoka genska nestabilnost, ki jeni bilo mogoře omiliti ne glede na uporabljeni postopek. Procedura prekinitve otcD-ORF1 gena, domnevne cilkaye je iyvedena v kromosomu bakterije S. rimosus. Medtem ko je prekinitev gena domnevne metilaye (otcY) iyvedena v S. lividans/pGLW101, ki vsebuje celotno skupino genov ya biosinteyo OTC na SCP2* playmidu. Predpostavljalo se je, da je domnevna ciklaya (otcD-ORF1) vpletena v ylaganje, cikliyacijo in aromatiyacijo domnevne poliketidne verige v biosinteyi OTC. Medtem naj bi produkt gena otcY iyvajal metilacijo C-6 ali pa C-4 N-poyicije molekule OTC. Iyvedeni sta prekinitvi genov, enega vpletenega v biosinteyo osnovnega skeleta molekule in drugega, ki, yelo verjetno sodeluje v naknadni modifikaciji Íe formiranega skeleta molekule OTC.Rekombinantna seva veř nista producirala OTC. Sev s prekinjenim otcD-ORF1 genom je produciral veřje Ǵtevilo novih spojin, ki jih prej ni bilo mogoře yaynati. Iykayalo se je, da posedujejo iyolirane spojine 9, 15 in 17 namesto 19 ogljikovih atomov. Tako multiencimski kompleks v odsotnosti otcD-ORF1 genskega produkta sintetiyira poliketidne molekule y reduciranim Ǵtevilom ogljikovih atomov. Sev S. lividans s prekinjenim otcY genom namesto OTC sintetiyira novo spojino. Struktura spojine, ki jo producira otcY mutanta Ǵe ni dolořena.
Type of material - dissertation
; adult, serious
Publication and manufacture - Ljubljana : [H. Petković], 1998
GS II 0000489863 glavno skladišče
GS II 489863 glavno skladišče
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