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  • Rekonstitucija aktivnosti steroidne hidroksilaze z encimskimi komponentami iz glive Rhizopus nigricans in goveje nadledvične žleze
    Makovec, Tomaž ; Breskvar, Katja ; Hudnik-Plevnik, Tamara
    The enzyme system which hydroxylates steroids at 11 alpha position in Rhizopus nigricans is composed of cytochrome P450 and NADPH-cytochrome P450 reductase. By using an in vitro assaysystem it was ... shown that this multienzyme system is located on fungal microsomes. In order to show the involvement of the reductase in hydroxylation we performed several reconstitution experiments with cytochromes P450 from bovine adrenal cortex. It was shown that only one NAPDH-cytochrome c reducstase, partly purfied from fungal microsomes, was active in reconstituted system with cytochrome P450 C21 from adrenal gland.
    Source: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Letn. 47, št. 3, sep. 1996, str. 293-297)
    Type of material - article, component part
    Publish date - 1996
    Language - english
    COBISS.SI-ID - 5048537

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