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Prevention of Campylobacter jejuni K49/4 adhesion to porcine small intestine cell line PSI cl1 using different plant extracts = Preprečevanje adhezije Campylobacter jejuni K49/4 na celično kulturo prašičjih črevesnih celic PSI cl1 z uporabo različnih rastlinskih ekstraktovŠikić Pogačar, Maja ...Namen: Da bi preverili inhibicijo adhezije C. jejuni, smo v poskusih in vitro testirali različne rastlinske ekstrakte na celičnem monosloju celic PSI cl1, in sicer ekstrakt tropin sorte Modri pinot ... (GSS), ekstrat oljčnih listov (OE) in timijana (TE) ter njegov preostanek po hidrodestilaciji eteričnega olja (TE-R) ter ekstrakt iz semen rastline Alpinia katsumadai (SEE) in njegov preostanek po hidrodestilaciji eteričnega olja (hdSEE-R). Želeli smo preveriti uporabnost odpadnega materiala in stranskih proizvodov agro-živilstva pri preprečevanju adhezije C. jejuni na celično linijo PSI cl1. Metode: Pred poskusom protiadhezijske učinkovitosti ekstraktov smo preverili njihovo protimikrobno delovanje ter citotoksičnost na celični liniji PSI cl1 in s tem določili koncentracijsko območje za testiranje protiadhezijske aktivnosti uporabljenih ekstraktov. Rezultati: Najučinkovitejša ekstrakta pri preprečevanju adhezije C. jejuni na celice PSI sta bila SEE in hdSEE- R, sledila sta TE in TE-R, ki sta do 30 % zmanjšala adhezijo bakterije na celično linijo. Najslabšo protiadhezijsko učinkovitost je imel ekstrakt GSS. Zaključek: Odpadni materiali (TE%R in hdSEE%R) ter OE, ekstrakt iz stranskega proizvoda agro%živilske industrije, so se pokazali kot zelo uspešni pri preprečevanju adhezije C. jejuni tudi pri zelo nizkih koncentracijah. Obetavni rezultati študije kažejo možnosti uporabe ekstraktov iz odpadnih rastlinskih materialov na različnih področjih, vključno z industrijo in skrbjo za zdravje ljudi in živali.Source: Acta medico-biotechnica : AMB. - ISSN 1855-5640 (Vol. 10, [no.] 2, 2017, str. 48-57)Type of material - article, component partPublish date - 2017Language - englishCOBISS.SI-ID - 512765496
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Šikić Pogačar, Maja | 29591 |
Klančnik, Anja | 22491 |
Smole Možina, Sonja | 07030 |
Mičetić-Turk, Dušanka | 02057 |
Source: Personal bibliographies
and: SICRIS
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