National and University Library, Ljubljana (NUK)
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Introduction of the comet assay for the evaluation of oxidative stress, antioxidant effects and environmental pollutants effects on animal cells = Vpeljava metode Comet za vrednotenje oksidativnega stresa, antioksidantnih učinkov okoliških polutantov na živalske celiceMarinšek-Logar, Romana, 1960-Poškodbe DNK v posamičnih sesalčjih celicah so lahko zgodnji pokazatelji številnih težav pri zdravju, prehrani, izpostavljenosti toksičnim snovem na delovnem mestu in v okolju nasploh. Poskus Comet ... je relativno enostavna in poceni metoda za merjenje tovrstnih poškodb. Temelji na določanju stopnje prekinitev v verigah celične DNK. Celice vklopimo v agarozni gel na mikroskopskih objektnikih in jih speremo, da odstranimo celične membrane, topno celično vsebino in jedrne histone. Pri elektroforezi fragmenti poškodovane DNK potujejo proti anodi. Pri epifluorescentni mikroskopiji obarvanih preparatov dobimo značilno sliko, ki je tehniki dala ime - skupek nepoškodovane DNK (glava) in rep kometa, ki ga tvorijo fragmenti poškodovane DNK. V članku poročamo o preverjanju metode Comet za vrednotenje oksidativnega stresa na prašičjih, mišjih in kokošjih krvnih celicah, prašičjih spermijih in piščančjih hepatocitah. Metodo Comet nameravamo uporabiti za kvantitativno vrednotenje v prehranskih poskusih domačih živali, kjer bomo preverjali oksidativne učinke nekaterih prehranskih snovi in vplive antioksidantov, in za preučevanje vpliva nekaterih onesnaževalcev okolja na živalske in človeške celice.Source: Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo. Zootehnika = Research reports [of the] Biotechnical Faculty University of Ljubljana. Agricultural issue. Zootechnica. - ISSN 1408-3434 (Letn. 74, št. 2, dec. 1999, str. 61-66)Type of material - article, component partPublish date - 1999Language - englishCOBISS.SI-ID - 912520
Author
Marinšek-Logar, Romana, 1960-
Topics
molekularna genetika |
DNK |
poškodbe |
oksidativni stres |
antioksidanti |
onesnaževanje okolja
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Marinšek-Logar, Romana, 1960- | 08404 |
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